How can unique molecular identifiers (UMIs) help to reduce quantitative biases?

ecSeq Bioinformatics · 05-28-2019, 04:17 AM

Maybe you have already heard about UMIs and how they can help to reduce errors and quantitative biases in your Next-Generation Sequencing (NGS) experiment.
But do you know how they work and how you should handle them bioinformatically after sequencing?

Here we will shortly introduce these molecular tags, which are added to the DNA fragments of your sample.

>> read full article

luc · 05-28-2019, 09:23 AM

Quote:

... we will shortly introduce these newly developed molecular tags ...

???

UMIs have been used since at least 2013, then sequenced on an old GAII:
"Exploring local immunological adaptation of two stickleback ecotypes by experimental infection and transcriptome-wide digital gene expression analysis."
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579235/
Please see the TrueQuant mentions in the paper.

ecSeq Bioinformatics · 06-04-2019, 03:27 AM

Thanks for the information, luc! We'll change it on the website.

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05-28-2019, 04:17 AM	#1
ecSeq Bioinformatics Senior Member Location: Leipzig, Germany Join Date: May 2012 Posts: 276	How can unique molecular identifiers (UMIs) help to reduce quantitative biases? Maybe you have already heard about UMIs and how they can help to reduce errors and quantitative biases in your Next-Generation Sequencing (NGS) experiment. But do you know how they work and how you should handle them bioinformatically after sequencing? Here we will shortly introduce these molecular tags, which are added to the DNA fragments of your sample. >> read full article __________________ ecSeq Bioinformatics is Europe’s leading provider of hands-on bioinformatics workshops and professional data analysis in the field of Next-Generation Sequencing (NGS). Last edited by ecSeq Bioinformatics; 06-04-2019 at 04:59 AM.

06-04-2019, 03:27 AM	#3
ecSeq Bioinformatics Senior Member Location: Leipzig, Germany Join Date: May 2012 Posts: 276	Thanks for the information, luc! We'll change it on the website. __________________ ecSeq Bioinformatics is Europe’s leading provider of hands-on bioinformatics workshops and professional data analysis in the field of Next-Generation Sequencing (NGS).