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Old 06-03-2013, 12:29 AM   #1
lbeltrame
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Location: Italy

Join Date: Nov 2011
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Default TruSeq Custom Amplicon - how does it fare?

Hello,

for one of our experiments we're thinking about using TruSeq Custom Amplicon for the selection of genes of interest (~50 in total with amplicon size of 250bp). Although with some difficulty, we managed to get the theoretical coverage close to 95%.

My question is: is anyone out there using this solution already? We've been early adopters of other solutions and we've been bitten severely (*awful* coverage). If you are using / have used TruSeq Custom Amplicon, what are your impressions? Was it OK, or are there some caveats one must know prior to using it?

Thanks!
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