Go Back   SEQanswers > General

Similar Threads
Thread Thread Starter Forum Replies Last Post
Case for why Genohub should be used for getting quotes and handling all your NGS work Genohub Service Providers 0 07-23-2016 12:26 PM
NGS Data Processing, Samtools, GATK, IGV Rez Bioinformatics 0 09-28-2012 06:36 AM
NAR: PileLineGUI a desktop environment for handling NGS genome position files. gonvader Literature Watch 0 06-09-2011 03:59 AM
Any good programs for handling solexa data? Burkholderia Introductions 1 08-13-2010 02:50 PM

Thread Tools
Old 01-30-2018, 02:24 PM   #1
Junior Member
Location: Devon

Join Date: Jan 2018
Posts: 1
Question Handling NGS data using IGV??

Good evening,

I do not work in bioinformatics, I have chosen a module in bioinformatics and completely out of my depth!
I have an assessment where I am given a reference bacterial genome in FastA format and a GFF file containing the annotations and the position of predicted genes.
I have illumine sequence reads generated by shotgun sequencing of genomes of several closely related bacteria.
The objective: identify interesting differences among the sequenced bacterial genomes!
I have no idea how to interpret this data. I started the module late due to personal reasons and I have 2 weeks to submit a poster with my findings!!

Can one or a few of you bioinformatics wizards help me out please?

Last edited by Alarrisa; 01-30-2018 at 02:30 PM. Reason: it was wrong
Alarrisa is offline   Reply With Quote
Old 01-30-2018, 04:02 PM   #2
Senior Member
Location: East Coast USA

Join Date: Feb 2008
Posts: 6,854

Can you tell us what you have learned as a part of this module? Do you know what the raw data looks like? What needs to be done with it in terms of QC/clean-up? Do you know what sequence alignment is with reference to NGS data? Are you limited to certain tools? Are you allowed to use online tools?
GenoMax is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 02:29 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO