emPCR on thermal cycler

[jbertsche]

Hi Everyone,

I know that the Roche manual says that the emPCRs can sit at 10 degC on the thermal cycler for up to 16 hours. I was wondering if any one has any experience with leaving them on for longer?

[crndy52]

I have started emPCRs and put them on the thermal cycler on Friday afternoon and left them until Monday morning when I started the breaking. I suppose this could make a difference, but I could not see any difference in my bead recovery rate or the run data when compared to other runs of the same samples.

[TonyBrooks]

I haven't left them for as long as a weekend, but I've left them 20 hours or so with no problems.