Hello,
I did some 454 sequencing, got the reads, manage to get some assemblies but as I am studying polyploids, I am interested in creating assemblies/alignments for each copy of the loci I sequenced. Is there a way to do this automatically? Maybe should I reassemble with very stringent parameters (if so how to tweak it smartly)? I am currently working with Geneious to handle the reads, do you know if it is possible with this software, or if I would need an other one?
Cheers,
I did some 454 sequencing, got the reads, manage to get some assemblies but as I am studying polyploids, I am interested in creating assemblies/alignments for each copy of the loci I sequenced. Is there a way to do this automatically? Maybe should I reassemble with very stringent parameters (if so how to tweak it smartly)? I am currently working with Geneious to handle the reads, do you know if it is possible with this software, or if I would need an other one?
Cheers,