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Old 05-17-2017, 03:36 AM   #21
jchoo
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Location: harbin,china

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hi,
Have anyone try analysis the error rate of per base in amplicons? We found lots of sites with error rate larger than 0.01, any idea why this happen?
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Old 10-03-2017, 02:43 PM   #22
Buckethead84
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Hi,

Has anyone gotten this protocol to work? I have a protocol that uses Phusion U MM (thanks to epistatic) that appears to work, but I haven't tried it without the end repair reaction yet. Do you think the end repair is necessary? I was also wondering anyone had any ideas if I could use the Qiagen Multiplex PCR kit to skip the end repair and the A-tail steps and proceed right from amplicon generation to ligation. Qiagen has published something last year showing that that the AmpliSeq Cancerv2 panel was compatible with the Qiagen Multiplex PCR kit.
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