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Old 09-24-2017, 07:52 PM   #1
aaagg
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Location: America

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Question Adapters Ion torrent

Hello everyone

I'm starting to work with the Ion torrent sequencer. I want to sequence fragments that I will amplify using primers forward and reverse.

My question is how do I add the Ion torrent A and P1 adapters to my amplified fragments. I already found the sequences of these adapters in what is published by Ion torrent.

żIs it necessary to add some recognition sequence to that said adapters can be pasted into a second PCR?
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Old 09-25-2017, 03:04 AM   #2
torben
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Quote:
Originally Posted by aaagg View Post
Hello everyone

I'm starting to work with the Ion torrent sequencer. I want to sequence fragments that I will amplify using primers forward and reverse.

My question is how do I add the Ion torrent A and P1 adapters to my amplified fragments. I already found the sequences of these adapters in what is published by Ion torrent.

żIs it necessary to add some recognition sequence to that said adapters can be pasted into a second PCR?
You can use fusion PCR primers (ie. primers with the A and P1 sequences included) directly in the first PCR (or in a second PRR run if you prefer): https://assets.thermofisher.com/TFS-...nMethod_UG.pdf

Alernatively, you can ligate adaptors to your PCR products: https://assets.thermofisher.com/TFS-...raryKit_UB.pdf
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