Hi , I am starting with tophat.
I have a paired end illumina HISeq RNA Seq dataset with two files:
SRR12331_1.fastq and SRR12331_2.fastq
should I reverse the reads files in SRR_2.fastq before mapping with tophat?
say I have a read from SRR12331_2.fastq:
TTTTGGG
should i feed tophat with :
GGGTTTT
THANKS!
I have a paired end illumina HISeq RNA Seq dataset with two files:
SRR12331_1.fastq and SRR12331_2.fastq
should I reverse the reads files in SRR_2.fastq before mapping with tophat?
say I have a read from SRR12331_2.fastq:
TTTTGGG
should i feed tophat with :
GGGTTTT
THANKS!
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