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Old 03-24-2014, 11:17 PM   #1
Nilaksha
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Default Visualize variants annotated with ANNOVAR

Hey all,

I'm developing a pipeline for NGS data analysis. I'm pretty much ok with the steps up to variant calling (I'm using GATK for that) and then I use the already built VCF file to convert it to an ANNOVAR compatible file for annotation. My problem is that, how can I incorporate ANNOVAR annotated files (there are several files I have got using Gene based/Filter based and Region based annotation) along with the VCF file in analysis. Can I add ANNOVAR annotated files as separate tracks along with VCF in visualisation programs like IGV?
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Old 03-25-2014, 06:08 AM   #2
TiborNagy
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Yes, you can. IGV can read a lots of data formats.
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Old 04-06-2014, 09:56 PM   #3
Nilaksha
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Quote:
Originally Posted by TiborNagy View Post
Yes, you can. IGV can read a lots of data formats.
I tried importing csv file obtained by table_annovar.pl script of ANNOVAR to IGV but had no luck. Is there any particular method that worked for you ?
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Old 04-07-2014, 12:28 PM   #4
blakeoft
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I'd like to know this too. I've tried opening file.exonic_variant_function and file.variant_function, and neither are visualized by IGV. The best thing I can think of is to add the appropriate headers to the Annovar output to make it look like a vcf. I guess some fields will have to be added as well.

EDIT: I just looked at the Annovar output again and it looks like it's going to need a lot more work than simply adding a header and some fields. But I think it might still be doable. I'll post a solution if I come up with one.

Last edited by blakeoft; 04-07-2014 at 12:32 PM.
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Old 04-08-2014, 03:41 AM   #5
TiborNagy
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I have worked with BED files, but IGV can read custom files:
https://www.broadinstitute.org/igv/custom_file_formats
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Old 04-08-2014, 07:44 PM   #6
Nilaksha
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Quote:
Originally Posted by TiborNagy View Post
I have worked with BED files, but IGV can read custom files:
https://www.broadinstitute.org/igv/custom_file_formats
Hey thanks a lot,

I guess I can work it out now/
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