What are considered "good" ranges for intensity and % alignment?
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I am working with at lab that will be doing pharmacogenetic assays using MiSeq. Getting the genotypes will be straightforward but copy number detection for CYP2D6 is a challenge on any platform because of confusion with CYP2D7. Does anyone know if there is a recommended way of estimating copy numbers with MiSeq?
Thanks,
Don Rule
Translational Software
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