ncRNA heatmap with next gen sequencing

[NicoBxl]

Hi,

I'm working on small RNA data from an Illumina GAII. After preprocessing the data, I've a matrix where each column is a sample and each line is a miRNA. The information is the number of reads.

Example :

--------- s1 s2 s3
miR1 10 50 0
miR2 20 0 100
miR3 30 20 60

how can i procduce a heatmap with this kind of data ?

Thanks,

N.

[KNS]

Soon as your data is in table format, I would always suggest you use R for the plotting. Check

http://sekhon.berkeley.edu/stats/html/heatmap.html

More sophisticated next gen packages for R can be found on the bioconductor (R) website http://www.bioconductor.org/

[NicoBxl]

ok to use R to produce a heatmap
but my question is more "how to normalize the data to produce a heatmap"