Tweet
what does pooled sequensing mean? who can tell me? thanks very much!!
-
-
Pooled sequencing is a method to find variations that are typical of a population. You mix equimolar concentration of DNA samples from individuals of a population and without any barcoding you sequence it. -
thanks for your answer! can you give me a detailed description of the pooled sequensing?Comment
-
It's as TiborNagy said. When you are sequencing, instead of separating different samples in a lane by barcoding you just put them in in the same concentrations so as to get a list of differences seen within the pool of samples obtained from the same population. When you are doing the downstream analysis of the data, you do not know which mutation/polymorphism came from which sample but you know that it exists in the population and given enough samples you can predict the frequency of it.Originally posted by bewlib View Post
hope that helps!Comment
-
You can read more about this technique in:
and here:
Comment
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment