Hi all,
One basic question; is it possible to share one flowcell (7 diferent lanes + control) for HiSeq with paired-end library samples (6 lanes) and one sample of small RNA library (1 lane) which doen't require paired-end rxn?
I guess that maybe the small RNA library needs to be run as a paired-end; could this to be done with the small RNA adapters used in this thread and the HiSeq platform:
Small RNA oligonucleotide sequences
RT Primer
5' CAAGCAGAAGACGGCATACGA
5' RNA Adapter
5' GUUCAGAGUUCUACAGUCCGACGAUC
3' RNA Adapter
5' P-UCGUAUGCCGUCUUCUGCUUGUidT
Small RNA PCR Primer 1
5' CAAGCAGAAGACGGCATACGA
Small RNA PCR Primer 2
5' AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA
Small RNA Sequencing Primer
5' CGACAGGTTCAGAGTTCTACAGTCCGACGATC
One basic question; is it possible to share one flowcell (7 diferent lanes + control) for HiSeq with paired-end library samples (6 lanes) and one sample of small RNA library (1 lane) which doen't require paired-end rxn?
I guess that maybe the small RNA library needs to be run as a paired-end; could this to be done with the small RNA adapters used in this thread and the HiSeq platform:
Small RNA oligonucleotide sequences
RT Primer
5' CAAGCAGAAGACGGCATACGA
5' RNA Adapter
5' GUUCAGAGUUCUACAGUCCGACGAUC
3' RNA Adapter
5' P-UCGUAUGCCGUCUUCUGCUUGUidT
Small RNA PCR Primer 1
5' CAAGCAGAAGACGGCATACGA
Small RNA PCR Primer 2
5' AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA
Small RNA Sequencing Primer
5' CGACAGGTTCAGAGTTCTACAGTCCGACGATC
Comment