Length read

pingu · 09-25-2015, 04:13 AM

Hello!
I am using 454 Roche Junior and I would be trim my primers. How much it must be length a read for be accepted before both trimming and after?
Thank you in advance

Brian Bushnell · 09-25-2015, 10:04 AM

That depends on what you're doing. For small RNA studies, for example, even 17bp is useful. For most other cases, you'd want longer reads. So, what's the experiment?

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09-25-2015, 04:13 AM	#1
pingu Member Location: milan Join Date: Jul 2015 Posts: 26	Length read Hello! I am using 454 Roche Junior and I would be trim my primers. How much it must be length a read for be accepted before both trimming and after? Thank you in advance

09-25-2015, 10:04 AM	#2
Brian Bushnell Super Moderator Location: Walnut Creek, CA Join Date: Jan 2014 Posts: 2,707	That depends on what you're doing. For small RNA studies, for example, even 17bp is useful. For most other cases, you'd want longer reads. So, what's the experiment?