Hi everyone.
Hope you can help me. I'm totally beginner in this Bioinformatics world, trying to understand (and analyze if possible) data from RNA-Seq experiment. The final goal is to asses differential expression.
To start, I wanted to import my raw sequences (.fastq) into R Bioconductor, following the sample workflow described on the website:
dataDir <- <...>
fastqDir <- file.path(dataDir, "fastq")
bamDir <- file.path(dataDir, "bam")
outputDir <- file.path(dataDir, "output")
where <…> indicates information provided by the user.
And here is where I get completely lost… Can anyone explain me what is the information I'm supposed to introduce??
Thank you in advance!!
Hope you can help me. I'm totally beginner in this Bioinformatics world, trying to understand (and analyze if possible) data from RNA-Seq experiment. The final goal is to asses differential expression.
To start, I wanted to import my raw sequences (.fastq) into R Bioconductor, following the sample workflow described on the website:
dataDir <- <...>
fastqDir <- file.path(dataDir, "fastq")
bamDir <- file.path(dataDir, "bam")
outputDir <- file.path(dataDir, "output")
where <…> indicates information provided by the user.
And here is where I get completely lost… Can anyone explain me what is the information I'm supposed to introduce??
Thank you in advance!!
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