Hi All,
I am wondering: Must we have at least two adapters to generate Pair-end Reads(Take Illumina Platform for example) ?
I am also not sure about using other Platforms.
I found this tread in SEQanswers below
"vinay052003" said " I am not sure about other technilogies, but for Illumina 5' end sequencing cycle starts right from the start (5' end) of the actual sequence. So adapters sequence contamination in the final read would be only on 3' end. " Do you agree?
Thanks a lot for any response!!
I am wondering: Must we have at least two adapters to generate Pair-end Reads(Take Illumina Platform for example) ?
I am also not sure about using other Platforms.
I found this tread in SEQanswers below
"vinay052003" said " I am not sure about other technilogies, but for Illumina 5' end sequencing cycle starts right from the start (5' end) of the actual sequence. So adapters sequence contamination in the final read would be only on 3' end. " Do you agree?
Thanks a lot for any response!!
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