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Old 07-31-2008, 12:52 AM   #1
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Default 454 amplicon sequencing - gene family with large number of paralogs

Thanks for your interests. The problem was solved. The paralogs can be treated as known variants, since the 454 amplicon analyzer supports this functionality.

As requested, I re-posted the question I asked earlier (I didn't save the exact copy, just try my best to rewrite the question):
I am studying the association of genetic variations within a gene family to certain phenotypes, and is planning to speed up the screening process with 454 amplicon sequencing. But I don't know if I should treat each paralog as a reference sequence or as known variants. The paralogs are highly similar at sequence level, with a few point mutations and small indels.

Last edited by qiwei; 08-04-2008 at 01:13 AM.
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Old 07-31-2008, 10:17 AM   #2
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Hi Wei,

In the future it would be great if you could leave your post here for others to reference, as someone will undoubtedly have the same question in the future.
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