Hi,
We would like to do differential expression analysis, we did a genome (Illumina + PacBIo) and a transcriptome (Kapa mRNA kit) but GC-rich genes are missing.
What is the best library prep for GC-rich genes? I am working with birds and some genes have a GC-content above 70%?
We are interested in genes which have low expression levels, apparently the rRNA depletion will not work well for our non-model organism (http://www.epibio.com/rnamatchmaker). What will you use?
Thank you for your answer.
Anne-Lyse
We would like to do differential expression analysis, we did a genome (Illumina + PacBIo) and a transcriptome (Kapa mRNA kit) but GC-rich genes are missing.
What is the best library prep for GC-rich genes? I am working with birds and some genes have a GC-content above 70%?
We are interested in genes which have low expression levels, apparently the rRNA depletion will not work well for our non-model organism (http://www.epibio.com/rnamatchmaker). What will you use?
Thank you for your answer.
Anne-Lyse
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