hi....
I got several samples'RNA-seq data(7) which have no replicate. these rawdata were converted into readscount of each simple.here are my problems:
Is there any good method to normalize this kind of data (withou any replication)?
Besides RPKM ,any other way to estimate expression level ?since I wanna perform network analysis next based on expression level info,and I 'm not sure that RPKM value could be accepted.
I tried edgeR with it's GLM method but it returned that replicated data are needed, I have no idea how to solve this now
I got several samples'RNA-seq data(7) which have no replicate. these rawdata were converted into readscount of each simple.here are my problems:
Is there any good method to normalize this kind of data (withou any replication)?
Besides RPKM ,any other way to estimate expression level ?since I wanna perform network analysis next based on expression level info,and I 'm not sure that RPKM value could be accepted.
I tried edgeR with it's GLM method but it returned that replicated data are needed, I have no idea how to solve this now
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