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It's possible that the double peak is caused by running too much sample for the chip (I'm guessing your image is from a Bioanalyzer chip or something similar). We've seen some seriously bizarre peaks on our Bioanalyzer (some double like yours and one that was actually a triple peak) that are remedied simply by re-running the sample at a 1:5 or 1:10 dilution.
Sometimes this eliminates all trace of a high MW smear as well, but typically I would guess that the presence of high MW fragments is due to over-amplifying the library. Do you usually amplify for 18 cycles? That is a little high in my experience (which, granted, I don't have tons of yet... ), but if it normally works for you than it might not be the issue.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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04-04-2024, 04:25 PM -
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