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Old 03-22-2018, 08:32 AM   #1
F.Lazaro
Junior Member
 
Location: Spain

Join Date: Jun 2017
Posts: 7
Question Buy NebNext Ultra DNA Library Kit components individually

Hello everyone,

Can someone explain why the NebNext Ultra DNA Lib Prep Kit is so expensive compared to the sum of the invidivual components?

NebNext Ultra DNA Library Prep Kit for Illumina: $535. 24 reactions
Contains:
-NEBNext® End Repair Module
-NEBNext® Q5® Hot Start HiFi PCR Master Mix
-Blunt/TA Ligase Master Mix
-NEBNext Ligation Enhacer

But:
NEBNext® End Repair Module: $85. 20 reactions
NEBNext® Q5® Hot Start HiFi PCR Master Mix: $90. 50 reactions
Blunt/TA Ligase Master Mix: $97. 50 reactions
Total: $272

Regards,

Fernando
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Old 03-26-2018, 06:58 PM   #2
luc
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Location: US

Join Date: Dec 2010
Posts: 343
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The NEBNext® End Repair Module is missing the A-tailing component (likely taq ploymerase).
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