I'm doing some library preps for DE analysis, and I'm curious if this kit, which selects for mRNA will also capture viral RNA? I'm hoping to do DE analysis between infected and uninfected samples so I'm hoping I can determine infected status from the sequences as well. Any idea?
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I do not know enough about viruses - hardly anything. Are there 3' caps ? (I am only aware of 5' caps. )
With the poly-A enrichment you can remove most of the rRNA (usually more than 90% of your total RNA sample) from your sample. If you can somehow selectively A-tail your viral sequences (and not also the rRNAs), this might be workable.
Very likely ribo-depletion protocols would be a good way to remove the rRNAs in your case.
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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