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Hello everyone,
can you please tell me how to resolve it .
as i am working on Stacks tool here i am trying to run pstack , cstack and sstacks separately.
so i am getting this error
can you please tell me how to resolve it .
as i am working on Stacks tool here i am trying to run pstack , cstack and sstacks separately.
so i am getting this error
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[root@bioinfo3-c indica]# pstacks -p 36 -t sam -m 3 -i 1 -f RG-1.sam -o ref_out/pstack/ Min depth of coverage to report a stack: 3 Model type: SNP Alpha significance level for model: 0.05 Parsing RG-1.sam Analyzed 64766 sequence reads; Identified 62825 unique stacks from those reads. Merged 63144 unique Stacks into 56715 loci. Identifying polymorphic sites and calling consensus sequences...done. Number of utilized reads 64766 Mean coverage depth is 6.76754; Std Dev: 11.7072; Max: 110 Writing loci, SNPs, alleles to 'ref_out/pstack/...' Error: Unable to open tag file for writing.