Hello everyone,
Starting from 10 ng of input DNA, we get around 200 ng total library selected between 200-400bp after 17 cycles of PCR.
For actual chip samples, we get around 10 to 30 ng total library after 15 cycles of PCR.
Are these yields somewhat in the expected range or is some step of the library preparation inefficient?
Starting from 10 ng of input DNA, we get around 200 ng total library selected between 200-400bp after 17 cycles of PCR.
For actual chip samples, we get around 10 to 30 ng total library after 15 cycles of PCR.
Are these yields somewhat in the expected range or is some step of the library preparation inefficient?