Hello,
Does any have strong experience and can give good advice regarding the optimal concentration of libraries for RNA-Seq?
I normally have worked with 30-50 ng/ul, but in the last set of samples, libraries were too small, of about 2-10 ng/ul.
I am worried if I would lost any non-abundant transcript and the preparations are biased.
Thanks for the comments
Cristina
Does any have strong experience and can give good advice regarding the optimal concentration of libraries for RNA-Seq?
I normally have worked with 30-50 ng/ul, but in the last set of samples, libraries were too small, of about 2-10 ng/ul.
I am worried if I would lost any non-abundant transcript and the preparations are biased.
Thanks for the comments
Cristina
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