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Old 07-23-2018, 03:13 PM   #1
NM1000
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Location: Houston

Join Date: Jul 2018
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Default Rockhopper Analysis (IGV Viewer)

Hello Everyone,

I would greatly appreciate if anyone can help me out with analyzing "Rockhopper IGV Viewer Results". We are trying to figure out what does the dip or downward spike (indicated by red arrow in the attached image) mean. Could it possibly mean that no reads were found during sequencing? Is it safe to correlate it to mRNA degradation or mRNA stability?

RNA-Seq was performed on Illumina Next Seq 500, and the RNA samples were extracted from gram-positive bacteria.

Thank you for your time and effort.
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