Posted these under introductions, hence they may have been overlooked
Questions for the community:
Are there established error or background read distributions that would allow me to attach a p-value to an observed read count?
We are planning to use spike-ins to use observed read-counts to estimate transcript copy number. Are any of you using spike-ins in your sequencing experiments? If so, how are you estimating amount of spike-in to add?
TIA,
Anjan
Questions for the community:
Are there established error or background read distributions that would allow me to attach a p-value to an observed read count?
We are planning to use spike-ins to use observed read-counts to estimate transcript copy number. Are any of you using spike-ins in your sequencing experiments? If so, how are you estimating amount of spike-in to add?
TIA,
Anjan