Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Galaxy tool to filter low mapping quality reads?

    Does anybody know if there is a way to remove low quality reads on Galaxy? it could be easily done using command line based bamtools and all I have to do is specify the map quality. Is there an equivalent version on galaxy? Or is there a published workflow that does it for you? Thanks.

  • #2
    there is a tool called filter FASTQ (Filter FASTQ reads by quality score and length) under GENERIC FASTQ MANIPULATION

    Comment


    • #3
      Galaxy filter quality help

      Hello,
      trying to use galaxy to perform NGS analysis.
      I have created a history that contains my datasets. Now when I go down to pull the filterQC application I dont see my datasets in the "library to filter". How do I pull my datasets into the stream?
      Tutorial shows how to get data from UCSC and also if I have a work flow created then I can do it, but without a workflow , if I have to just use one tool to do the job how do I get my datasets to show up for an operation?
      Thanks for ur time
      geneart

      Comment


      • #4
        filterQC

        Before using Filter FastQ you must use the FASTQ Groomer.

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Current Approaches to Protein Sequencing
          by seqadmin


          Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
          04-04-2024, 04:25 PM
        • seqadmin
          Strategies for Sequencing Challenging Samples
          by seqadmin


          Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
          03-22-2024, 06:39 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, 04-11-2024, 12:08 PM
        0 responses
        18 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 10:19 PM
        0 responses
        22 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 09:21 AM
        0 responses
        17 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-04-2024, 09:00 AM
        0 responses
        48 views
        0 likes
        Last Post seqadmin  
        Working...
        X