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Old 05-23-2013, 01:28 AM   #1
stan1991
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Location: Stevenage

Join Date: Jan 2013
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Default DNA extraction of Sputum samples for Metagenomic project

Hi everyone,

I am carrying out a metagenomic project on a number of sputum samples. I have tested a protocol for 16S metagenomics on the Illumina MiSeq with a small sample of known species, which I extracted the DNA from individually. I haven't tried the DNA extraction step yet on a sample containing multiple bacteria yet.

The sputum samples have been obtained by glass bead processing and DTT processing. I have already order the Qiagen QIAamp UCP Pathogen Mini Kit and some samples of the MoBio UltraClean Microbial DNA Isolation Kit. I will be testing these kits soon, but I was just wondering whether anyone else has tried extracting prokaryotic DNA from sputum samples before and has any recommendations? Or if anyone has some advice they could give me on extraction kits?

Thanks for reading and any replies,

Stan
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Old 11-26-2013, 04:25 PM   #2
Nirajlincoln
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Location: New Zealand

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Quote:
Originally Posted by stan1991 View Post
Hi everyone,

I am carrying out a metagenomic project on a number of sputum samples. I have tested a protocol for 16S metagenomics on the Illumina MiSeq with a small sample of known species, which I extracted the DNA from individually. I haven't tried the DNA extraction step yet on a sample containing multiple bacteria yet.

The sputum samples have been obtained by glass bead processing and DTT processing. I have already order the Qiagen QIAamp UCP Pathogen Mini Kit and some samples of the MoBio UltraClean Microbial DNA Isolation Kit. I will be testing these kits soon, but I was just wondering whether anyone else has tried extracting prokaryotic DNA from sputum samples before and has any recommendations? Or if anyone has some advice they could give me on extraction kits?

Thanks for reading and any replies,

Stan
Hi Stan, have you found suitable method for extracting DNA samples from sputum?. I am also planning to do extract DNA from feces. Can you please let me know if you found good approach and can you please tell me which method is best to analyse metagenome in terms of costs? nirajmohan@lincolnuni.ac.nz, thanks
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