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Old 06-30-2013, 04:29 PM   #1
Amielynn
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Location: Portland, Oregon

Join Date: Jul 2011
Posts: 2
Default Target prediction between complementary RNA-seq and Small-RNA-seq data sets

Hi everyone-

Short background: I have Illumina sequence data on mRNA and microRNA from the same samples. Theoretically these two separate lists of sequences should contain complimentary microRNAs and mRNAs. I have no reference genome for my species however many of my microRNAs match to already annotated sequences from related species on miRBase along with a few potential novel microRNAs.

Question: Does anyone know if it is possible to datamine these two profiles and predict mRNA-microRNA targets from de novo assembled transcriptome data? There seem to be many programs that utilize reference transcriptome data but I am not interested in that since I have assembled the transcriptome for my species de novo from the mRNA RNA-seq data.

I'm not sure if I can use de novo assembled transcriptome data for microRNA target prediction through already existing programs; I currently have a subscription to CLC genomics workbench which appears unable to do so. This could potentially require the development of a custom script... Any information would help!

Thanks!

arom2
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de novo transcriptome, microrna, rna-seq, target prediction

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