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Old 02-07-2013, 08:29 AM   #1
theward
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Default qPCR Library Quantification

From reading other threads most people prefer the KAPA kits to run qPCR for library quantification. Can anyone tell me why these kits are superior to the SYBR green kits as my understanding is they're more expensive? If it to do with the standards being normalised could you not just use your own library to generate a standard curve with the SYBR green kits?
Any advice most welcome!
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Old 02-07-2013, 12:04 PM   #2
microgirl123
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We use the KAPA kit for convenience and because we know it works! The standards are premade, the sizing conversion is easy, and I've never had it fail.
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Old 02-07-2013, 12:23 PM   #3
GW_OK
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Same here. It's incredibly easy to use and always gives reproducible results.
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Old 02-13-2013, 08:20 AM   #4
theward
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Can I ask if you are still sizing the libraries with the bioanalyser/tapestation? If not, how are you sizing the libraries?
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Old 02-13-2013, 12:03 PM   #5
GW_OK
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Tapestation for size and initial quantification in the ballpark range. Kapa kit for actual dilution calculations.
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Old 02-13-2013, 12:06 PM   #6
kwaraska
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Same for us. We use tapestation for quant and size to normalize so it ends up on our standard curve.

Last edited by kwaraska; 02-13-2013 at 12:07 PM. Reason: spelling
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