I have been using CLC Genomics for RNA-seq analyses. I am using the gaussian-based tests in CLC Genomics to detect differentially expressed genes but I am interested in using Bioconductor/R packages, such as DESeq. Can I use the exported bam/sam files produced from CLC's RNA-seq mapping tool in DESeq? Or do I need to use bam/sam files from another mapper such as TopHat? Additionally, can I use the bam/sam files from CLC as input into the HTSeq-count program? Thank in advance for you help.
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...-
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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