Hi all,
This may be a stupid question, but I was wondering if anyone knew what sort of mRNA recovery rate one generally expects from the Sera-Mag beads that come in the Illumina mRNA-seq library kit. I'm getting really low recovery rates, on the order of 1% of the original transcript present, but I don't know if that's normal or not.
This may be a stupid question, but I was wondering if anyone knew what sort of mRNA recovery rate one generally expects from the Sera-Mag beads that come in the Illumina mRNA-seq library kit. I'm getting really low recovery rates, on the order of 1% of the original transcript present, but I don't know if that's normal or not.
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