weird 454 reads

[eoh001]

Hello
I have a weird 454 result produced by MID and DSN normalization.
The mRNA reads started with 'key(4bp)+MID(10bp)+cDNA library primer (20~25bp) sequence from 5' end. The reads are supposed to included key+MID, not cDNA library primer. I feel like that there seemed to be something wrong with cDNA library construction. Below is the sample reads.
Are they normal?


>GRMETAB01A1BYK
tcagACGAGTGCGTAAGCAGTGGTATCAACGCAGAGTGGGAGAATCTCGAACCACATCTCTGCCCGTACCTAGCaacctaactctggtcgtnaagagctgntcncctcccncctcctntgtgtgtactactacgctcttntagacagcacgacgacacacggaggtaggagnn
>GRMETAB01AK1L7
tcagACGAGTGCGTAAGCAGTGGTATCAACGCAGAGTGACTGAAACACAATTCATTTCTGACTGACATTacctttctgtcaactagacggtggcaccacaaataataccggtctttgtgcttgtctcttctagatgganggtttgttaaggactcttgttgtcattactttaaaaagaagtaataaaggattttaaagtaacgaaaaaacgaaaagaaaaactaaaactaaacgtcgtgtagtacactacacgtctctgagagcacgacgacacacggaggtaggngnnnnn
>GRMETAB01BC7E6
tcagACGAGTGCGTAAGCAGTGGTATCAACGCAGAGTACGGAGGTGTTGAAGaagattgaatcaagaactaacagccaaagaagaagaagaagaagaagacagaagaggagcagaagaaactaagacacagattcaagaatctcaggtaacatccaccaacaaggaacggacggttcctaacgacgggagtgagaactaagacaagaaatactcaagaccactggaggaggctaaagacagagcgagaaaacgaaagaacagaacaagctgagctgagactgccaacggacacacagggagtaggnnnn
>GRMETAB01EOICN
tcagACGAGTGCGTAAGCAGTGGTATCAACGCAGAGTACTGAGGGAAGTAGTTGTGTTGGCTGAGTTGTCAGTCAAGCGAATTCCGCTACTCTGCGTTGATACCACTGCTctgagactgccaaggcacacagggataggnnnnnnnnnn
>GRMETAB01DR31L
tcagACGAGTGCGTAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTNTTTNTTTAttataactaactaactatatatattattattatatatatctctactactactactactatatactactatatatatactactactacaccacacacgtagtagtagtacgtactactactactacgagagagagagagagacgagtaggtagtacgtacgtacgtacgacgacgtactactaactaactaactacgtacgacgacgacgacgacgacgacgtacgtacgtaccgtaccgtaccgtaccgtaccgtaccgacctacctaacctacctaacctactactactacgtacgacgacgacgacgacgacgacgagagagngnnnn
>GRMETAB01A4CCL
tcagACGAGTGCGTAGCAGTGGTATCAACGCAGAGTTACTTTTTTTTTTTTTTTTTTTTTNttttnttttnttttnttttnttttntttatttatttatttatttatttatttattattattatatatatatatatatatatatatatatatatatatagtatatatatatatatatatatatatatatatatatatatatagtatagtagtagtacgtacgtacgtacgtacgtacgtacgtacgtacgtacgtacgtacgtacgtacgtacgtacgacgacgacgacgacgacgagagagagagngnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
>GRMETAB01BL4UE
tcagACGAGTGCGTAAGCAGTGGTATCAACGCAGAGTACTTTNTTTNTTTCTTTCTTTCTTTCTTTCttctctactatctacaanagacacacactctctctcgtctcgtcgtacgtacgtacgtcgacgacgacgacgacgacgacgagagagngnnn
>GRMETAB01BWBZ9
tcagACGAGTGCGTAAGCAGTGGTATCAACGCAGAGACAGCCAAGTCATTGCCAGACACATACAAAGATAAGCCCACGTActtacngcgttgataccacgtcggcttctgaggacgttggaccaaagggacgacctaacctagggggaattaggggngcncncnngagagaggngcngtnttnanntgngnngagangcacagatacgctcgcagacgtgggttcggtccgacgacccgtaacggtaacctacaacgcttcctttaggttatagtaattaaatttagtacttatttcgttcggacggacgaacgaaaaacgggaaaccgtaacgtaccaccacgtacctactaggacaaacaactacttcttcgtcgtctagtaggacgagggaggacgtacgtaggtagtttattattaattacttctagaagacacagagacgacgaccaacgtactattcgaccgaacgaacaacaactaaacaaaacgaaacgtaaaccgacccgtaacgtacttacgtcgtcgttacgtaactaacctactcgtcttcgtacgacgacgtacgaccgaacggacgacgaacggaagggaaggtaggagnnnnn
>GRMETAB01C584Q
tcagACGAGTGCGTAAGCAGTGGTATCAACGCAGAGTACAGGGGTGTTTACAAGAGATGGTTTGCAATGCAGACTaagcagaaaatttagtagngtgatggataggaaaccaaaccaacagcccccgtacctctncgtgtatacacctgctcttcgagactgcacggacgacacaggggagtagngnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
>GRMETAB01AOKGH
tcagACGAGTGCGTAAGCAGTGGTATCAACGCAGAGTACGTTTNTTTNTTTTTTTTTNTTTTTTNTTTTTNttttnttttntttttattttattttattttattttattttattttatttatttatttatttatttatttatttatttattacttacttattagtagtagtagtagtacgtagtacgtacgtacgtacgtacgtacgtacgtacgtactacgtacgtacgtacgtacgtacgtactactactactactactactactacgtacgtacgtacgtacgtacgtacgtacgtacgtacgtacgtacgtacgtacgacgacgacgacgacgacgacgagacgacgagagannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
>GRMETAB01D7O01
tcagACGAGTGCGTAAGCAGTGGTATCAACGCAGAGTACGGGAATTATGACATGCAccgatttacatcccncctctcngtcgtatanacctatctccttcagaagtnttctacgagaaccaacctaggnggacgagatntctcntacgtcttgtacganctacctcgngnnntcncntctcgttgtggtggttcttacgtcgttcgttgtagttgtaagtaacgtacgtgagagaagacgtacgtacgactacctacgtacgtacaactaaagttaaagttaaattaaaaagtaaaaagttaaagtttaagtttaccgttaccgtacgtaggtaaggaaaggaaaggaacgaacgtaaacgtttaaccgtttaaaccgtaacgaaccgaacccgaaaccgtaaacggtaaaaacggaaaaaccgaaaaccgaaacgtaagtacgaacggaaacggtaacgtaacggaaggaaggaacgaaaccgtaaaacgttaaacgttaaacgtaaacgtaaaacgtaaacgtaaacgtaacctaaccgtaacgtaagttacgtacgtagtagtaacgtaacgaaacgaaccgtaaccgtaaacgtaaactaacgtaacgtaaccgttaccgttaccgttacccgttaacccttaaaccccttaacccttacgttacgtaacggtaacggtaaaggttaaagttaagtaacgtaacgtacgtacgtaaccgtaaccgttaaccgttaaccttaaccccgttaccccgttacccgttaccgttacggttacgttacgtaaccgtaaccgtaccgttaccgttaccgttacgtacgtacg
>GRMETAB01AHC9E
tcagACGAGTGCGTAAGCAGTGGTATCAACGCAGAGTAGGTGATGGAAACCTTTGGTAGACTCTTCTAAGTGCACGTGCAGATTTTCTCTTACCATGTATTTTACTTAAACAAATTTGTTTGTTATTTagtccacccgcctccttcttctacacacctctcgttccttcctaccgccactacttaacttagttgtcggttccccgttaccttcctaccgtccgttntgtcgtactanaccttcctgttgaccttgacgaagccacgaccgaagggactaggaggaggnngnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn

[GW_OK]

TTTNTTTNTTTTTTTTTNTTTTTTNTTTTTN looks like a poly A primer, broken up to avoid a huge homopolymer run.

I'll also bet that the >GRMETAB01A4CCL read hit that wall of T's and died immediately, hence 'tatata'.

[eoh001]

TTTNTTTNTTTTTTTTTNTTTTTTNTTTTTN looks like a poly A primer, broken up to avoid a huge homopolymer run.

I'll also bet that the >GRMETAB01A4CCL read hit that wall of T's and died immediately, hence 'tatata'.

I still wondering whether the reads including poly-A primer are common or not. I mean which one is normal read?

(1)
tcagACGAGTGCGTAAGCAGTGGTATCAACGCAGAGTGGGAGAATCTCGAACCACATCTCTGCCCGTACCTAGCaacctaactctggtcgtnaagagctgntcncctcccncctcctntgtgtgtactactacgctcttntagacagcacgacgacacacggaggtaggagnn

(2)
tcagACGAGTGCGTGGGAGAATCTCGAACCACATCTCTGCCCGTACCTAGCaacctaactctggtcgtnaagagctgntcncctcccncctcctntgtgtgtactactacgctcttntagacagcacgacgacacacggaggtaggagnn