Does anyone have a standard or threshold of % rRNA they require in order to proceed with mRNA library prep?
In the past I've only proceeded with Illumina library prep after SeraMag polyA selection if I see 2% or less rRNA contamination on the bioanalyzer mRNA pico trace.
I'm currently using the Dynabeads polyA kit to obtain mRNA for ScriptSeq library prep. I've been getting anywhere from 0-7% rRNA with the same protocol (all samples had high RINs). I'm wondering if the 2% threshold is too stringent.
In the past I've only proceeded with Illumina library prep after SeraMag polyA selection if I see 2% or less rRNA contamination on the bioanalyzer mRNA pico trace.
I'm currently using the Dynabeads polyA kit to obtain mRNA for ScriptSeq library prep. I've been getting anywhere from 0-7% rRNA with the same protocol (all samples had high RINs). I'm wondering if the 2% threshold is too stringent.
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