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Thread | Thread Starter | Forum | Replies | Last Post |
Two emulsion PCRs?? | dmrodz | 454 Pyrosequencing | 2 | 06-30-2011 08:16 AM |
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emulsion pcr - one template-fragment per bead? | Triticum | 454 Pyrosequencing | 1 | 11-30-2009 11:31 AM |
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#1 |
Member
Location: Maryland Join Date: Jun 2011
Posts: 16
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Hello all,
I performed my first E80 yesterday. I had several bubbles form around the upper triangle piece when I was transferring the emulsion to the amplifier pouch. Despite nearly an hour of trying I was not able to eliminate them all. After amplification I can see a small number of broken reactors in the form of speckling at the corner of the bag at the bottom of the pouch ![]() Also if you have experienced this does it kill your run? I will post once I know the results of my wfa... the e10 scale I performed on this library looked beautiful though. |
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#2 |
Member
Location: Maryland Join Date: Jun 2011
Posts: 16
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ok so I now know why no one replied wfa looked ok.
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Tags |
amplification, ezbead |
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