Go Back   SEQanswers > Applications Forums > Genomic Resequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
can we know target region of exome without bed file ? cjo4m4fm Bioinformatics 3 10-01-2013 12:19 PM
On/off target rate for whole exome data chris Bioinformatics 5 01-21-2013 01:56 AM
Is it necessary to trim the low quality ends before analyzing exome seq data? gary Bioinformatics 0 10-11-2012 08:00 PM
BAM2MPG Exome Target File MolecularToast Bioinformatics 0 07-17-2012 07:49 AM
Estimate on-target coverage for exome data pravee1216 Bioinformatics 10 04-12-2012 12:20 PM

Thread Tools
Old 05-31-2015, 01:48 AM   #1
Junior Member
Location: Beijing,China

Join Date: May 2013
Posts: 6
Default Trim to target in exome-seq

Dear all,
I am now analyzing some downloaded WES data to find SNPs and there certainly could have reads map to introns.
My questions are:
Should I remove these reads map to intons or just keep them? What difference does it make?
I plan to use USCS RefSeq Gene as annotation of exon boundaries, are there any notable difference between this annotation and exon array used to purify exome during experiment?

zzy is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 01:38 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO