I am curious about the directions for the Zymo Genomic DNA Clean & Concentrator recommended by the Illumina kit. It says to add 2 volumes of the DNA Binding Buffer to the tagmentation reaction and "mix thoroughly." What sort of mixing? flicking the tube? Pipetting? I am assuming that flicking the tubes is the safest thing to do here since I don't want to shear the DNA at this point.
Many thanks to anyone with experience and advice.
Gina
Many thanks to anyone with experience and advice.
Gina
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