Dear all,
I've been asked to help with the bioinformatics for a methylation experiment - human cancer cells infected with two differential bacterial strains from the same species. Within a sample there will be infected and non-infected cells, a methylation profile of the normal human cells and a methylation profile of the infected cells, are there any methylation techniques available that would be able to pick this up? I have alot of experience with HM450 but that just gives a consensus result. What about Methyl-seq?, would it work aligning sequence to a bacterial genome and to a human genome? Any advice greatly appreciated.
I've been asked to help with the bioinformatics for a methylation experiment - human cancer cells infected with two differential bacterial strains from the same species. Within a sample there will be infected and non-infected cells, a methylation profile of the normal human cells and a methylation profile of the infected cells, are there any methylation techniques available that would be able to pick this up? I have alot of experience with HM450 but that just gives a consensus result. What about Methyl-seq?, would it work aligning sequence to a bacterial genome and to a human genome? Any advice greatly appreciated.
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