How can I know if the adapter ligated?
For three of my samples (we are 6-plexing) I forgot to anneal the adapters, however all the samples turned out really well and would be ready for a lane in Illumina. The other three samples are ligated with adapter we had a for a long time and have been working perfectly.
Is there any way to find out if the adapters are ligated anyways before running a lane on Illumina?
Thank you very much.
For three of my samples (we are 6-plexing) I forgot to anneal the adapters, however all the samples turned out really well and would be ready for a lane in Illumina. The other three samples are ligated with adapter we had a for a long time and have been working perfectly.
Is there any way to find out if the adapters are ligated anyways before running a lane on Illumina?
Thank you very much.
Comment