Tweet
Please
Somebody could help me ?
We are used to demultiplex Truseq data....but Nextera is not working ?
Thanks
Somebody could help me ?
We are used to demultiplex Truseq data....but Nextera is not working ?
Thanks
-
-
What do you mean by "not working"?
If you have access to the bcl files, you should be able to demultiplex using the CASAVA command configureBclToFastq.pl and a compatible spreadsheet
-
Are you doing the de-multiplexing on the HiSeq itself or off-line using CASAVA?
You will have to provide some additional details in order for us to be able to help.Comment
-
Is necessary to change the configuration of CASAVA to demultiplex Nextera data? Is the first time we are analysing Nextera data...before that we analyzed TRuseq data.Originally posted by GenoMax View Post
We are doing off-line using CASAVA
Thank you very muchComment
-
CASAVA should not need any changes. What is exactly happening? Are you getting an error or is everything going into the "Undetermined" bin?Comment
-
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
Comment