Agilent Bioanalyser X axis doesn't size bands

Sergioo · 01-24-2014, 12:50 AM

I raise this thread because I got difficult getting a way to change the X axis of Agilent Bionalyzer.
In fact, this axis is showing up in seconds and it won't change to size bands.
I saw a related post by pmiguel here http://seqanswers.com/forums/archive...hp/t-3338.html, but there is no answer there on how solve this.
Please note that both marker were well recognized and samples as well (samples has bigger than expect bands though).
I would like to hear from you a way out this.
Thanks

microgirl123 · 01-24-2014, 07:06 AM

Just to check, you are using the toggle button to change the seconds to size? And it is not working? Is this all the time or just for one run? I have done one run that will not change - everything looks perfect, it just won't switch from seconds to size. If you use the region table, and put regions around the peaks you are interested in, you can tell the size from the table.

Sergioo · 01-25-2014, 02:33 AM

Quote:

Originally Posted by microgirl123

Just to check, you are using the toggle button to change the seconds to size? And it is not working? Is this all the time or just for one run? I have done one run that will not change - everything looks perfect, it just won't switch from seconds to size. If you use the region table, and put regions around the peaks you are interested in, you can tell the size from the table.

I did resolved it using the region table.
Thanks

kwaraska · 02-03-2014, 09:27 AM

Usually if it won;t toggle it is because of an error in one lane.

If you can remove that error, even if it means losing that sample, you can recovere the rest of the run.

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
mRNA on Bioanalyser	dobbr493	Sample Prep / Library Generation	11	04-25-2012 07:09 AM
Agilent Bioanalyzer refuses to size bands	pmiguel	Sample Prep / Library Generation	13	03-09-2012 03:29 AM
RNA quality (bioanalyser) for agilent microarray	rabiecqa	Sample Prep / Library Generation	10	04-15-2011 03:24 AM
Agilent SureSelect for SOLiD - insert size and qPCR for enrichment QC	OCD Overload	Sample Prep / Library Generation	0	03-20-2011 05:37 PM
Insert size with Agilent SureSelect	sdavis	Sample Prep / Library Generation	12	05-14-2010 10:39 AM

01-24-2014, 12:50 AM	#1
Sergioo Member Location: Japan Join Date: Oct 2013 Posts: 29	Agilent Bioanalyser X axis doesn't size bands I raise this thread because I got difficult getting a way to change the X axis of Agilent Bionalyzer. In fact, this axis is showing up in seconds and it won't change to size bands. I saw a related post by pmiguel here http://seqanswers.com/forums/archive...hp/t-3338.html, but there is no answer there on how solve this. Please note that both marker were well recognized and samples as well (samples has bigger than expect bands though). I would like to hear from you a way out this. Thanks

01-24-2014, 07:06 AM	#2
microgirl123 Senior Member Location: New England Join Date: Jun 2012 Posts: 200	Just to check, you are using the toggle button to change the seconds to size? And it is not working? Is this all the time or just for one run? I have done one run that will not change - everything looks perfect, it just won't switch from seconds to size. If you use the region table, and put regions around the peaks you are interested in, you can tell the size from the table.

02-03-2014, 09:27 AM	#4
kwaraska Senior Member Location: Boston,MA Join Date: Nov 2008 Posts: 122	Usually if it won;t toggle it is because of an error in one lane. If you can remove that error, even if it means losing that sample, you can recovere the rest of the run.