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Hi all,
We have made the cfDNA library like this with a lot of zigzag peaks, and the library output amount is only half of normal，about 1000ng from ~20ng extracted cfDNA through 8 round PCR cycles. We are using KAPA library preparation kit. We following performed library capture using the library with a panel, following sequencing only got far below expected data output.
we repeat the preparation procedure using the original cfDNA with normal procedure and both library output and peak profile are normal. Have you ever met this condition? Many thanks！
We have made the cfDNA library like this with a lot of zigzag peaks, and the library output amount is only half of normal，about 1000ng from ~20ng extracted cfDNA through 8 round PCR cycles. We are using KAPA library preparation kit. We following performed library capture using the library with a panel, following sequencing only got far below expected data output.
we repeat the preparation procedure using the original cfDNA with normal procedure and both library output and peak profile are normal. Have you ever met this condition? Many thanks！
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