Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Cuffmerge error (GFaSeqGet): subsequence cannot be larger than

    Hi all. I am doing some differential expression calculations using the Tuxedo pipeline. I've take all reference files (GTF, reference genome) from ensembl GChr38.
    So far, I've run Tophat and Cufflinks with no problem. When I try to run Cuffmerge on the data, I get the following error:

    Code:
    Error (GFaSeqGet): subsequence cannot be larger than 632
    Error getting subseq for ENST00000335137 (69091..70008)!
            [FAILED]
    Error: could not execute cuffcompare
    In all my reference files, as well as the GTF files generated by cufflinks, the length of the transcript is 917 bases (69091 - 700008). Where does the "632" in the error message come from? Can anyone offer some insight into how to solve this?

    I've had a look at some other posts with similar issues, but have not been able to solve it. Here are my parameters:

    Code:
    tophat --no-coverage-search --no-novel-juncs -T -p 20 -o th-out --transcriptome-index Homo_sapiens.GRCh38.80 -G Homo_sapiens.GRCh38.80.gtf Homo_sapiens.GRCh38.80 file1.fastq file2.fastq
    
    cufflinks -p 20 -G Homo_sapiens.GRCh38.80.gtf  -o cl-out th-out/accepted_hits.bam
    
    cuffmerge -o cuffmerge -g Homo_sapiens.GRCh38.80.gtf -s Homo_sapiens.GRCh38.80.fa -p 20 assemblies.txt
    Thanks for any help

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin


    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
    Yesterday, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
39 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
41 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
35 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
55 views
0 likes
Last Post seqadmin  
Working...
X