Hi folks,
In general, RNAseq data gets reads predominantly on internal regions of a transcript, and the 5' and 3' ends are somewhat underrepresented. Am I right? If yes, why is this seen? I mean, which step of library construction causes this.
Any citations/references will be really helpful.
Thanks.
In general, RNAseq data gets reads predominantly on internal regions of a transcript, and the 5' and 3' ends are somewhat underrepresented. Am I right? If yes, why is this seen? I mean, which step of library construction causes this.
Any citations/references will be really helpful.
Thanks.