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  • Combined Small RNA and mRNA Library Prep

    The NEXTFLEX® Combo-Seq™ mRNA/miRNA kit enables the user to generate mRNA and small RNA libraries in a single workflow using 5 ng – 100 ng of total RNA inputs, without requiring upfront rRNA depletion or poly(A) selection. The kit utilizes patented and patent-pending technology allowing the construction of a library from both mRNA and small RNA from a single input. Like the NEXTFLEX® small RNA-seq kit v3, the NEXTFLEX® Combo-Seq™ mRNA/miRNA library preparation kit utilizes adapters with randomized ends to greatly reduce bias compared to standard small RNA library preparation protocols. This allows a more accurate representation of small RNA in the starting material. Adapter-dimer reducing techniques built into the protocol enables gel-free workflow from inputs as low as 5 ng of total RNA. As a result, the kit is streamlined from start-to-finish and automated on the Sciclone® G3 NGS/NGSx workstations. Additionally, unique dual index barcode primers, included in the kit, prevent sample mis-assignment on Illumina® NovaSeq®, MiSeq®, and HiSeq® 2000/2500 platforms.
    For research use only. Not for use in diagnostic procedures.

Latest Articles

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  • seqadmin
    Techniques and Challenges in Conservation Genomics
    by seqadmin



    The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

    Avian Conservation
    Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
    03-08-2024, 10:41 AM
  • seqadmin
    The Impact of AI in Genomic Medicine
    by seqadmin



    Artificial intelligence (AI) has evolved from a futuristic vision to a mainstream technology, highlighted by the introduction of tools like OpenAI's ChatGPT and Google's Gemini. In recent years, AI has become increasingly integrated into the field of genomics. This integration has enabled new scientific discoveries while simultaneously raising important ethical questions1. Interviews with two researchers at the center of this intersection provide insightful perspectives into...
    02-26-2024, 02:07 PM

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