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  • Mapping to SOLiD reads to draft genome

    I am presently involved in hybrid assembly and want to map my SOLiD reads against ROCHE contigs (length more than 1000bp). I approach is to map the reads with corona_lite, but facing problem with the cmap file. I found a small paragraph regarding this particular situation in corona_lite documentation but it is not that illustrative. Usually for mapping against human genome we take cmap as follows:

    1 1 path to reference for each chr. path to the double encoded file

    But for a draft genome i am confused. it tried with this following approach but i failed. i present cmap that i tried was:

    1 contig0001 path to the multifasta contig file

    Any help and guidance will be appreciable.

    with regards,
    saha

  • #2
    Either make a very large cmap file with one line per contig file. Or put all of the contigs into one file and separate them by 'N's; that is the file will look like one large sequence. I usually do the latter. If you are clever with how many N's you use to separate each contig then you can get information from the mapping file; e.g., map to bases 1-1323 means contig#1, bases 1500-2300 contig #2, etc.

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