Hi there,
I am a beginner in sequencing field, I have several silly questions hopefully someone can give me some instruction.
I just got the our sequencing data from Solexa 1G analyzer which gave us s_*_000*_seq.txt and s_*_000*_prb.txt
First question:
Why the length of each read is 152bps (76x2) instead of 76bps (38x2)?
Second question:
What is the first process I should do? I already convert raw text file into .fasta format, should I split the 152bp read into 2 short reads with 76 bps or even shorter?
Third question:
For data input for Maq, we need to convert reads from fastq to bfg. But I only have s_*_000*_seq.txt and s_*_000*_prb.txt in hand, which software can I use for this step conversion or I can do this by Matlab?
Thanks your time helping me answer these questions.
I am a beginner in sequencing field, I have several silly questions hopefully someone can give me some instruction.
I just got the our sequencing data from Solexa 1G analyzer which gave us s_*_000*_seq.txt and s_*_000*_prb.txt
First question:
Why the length of each read is 152bps (76x2) instead of 76bps (38x2)?
Second question:
What is the first process I should do? I already convert raw text file into .fasta format, should I split the 152bp read into 2 short reads with 76 bps or even shorter?
Third question:
For data input for Maq, we need to convert reads from fastq to bfg. But I only have s_*_000*_seq.txt and s_*_000*_prb.txt in hand, which software can I use for this step conversion or I can do this by Matlab?
Thanks your time helping me answer these questions.
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