Hello,
I am trying to trim low quality bases from a .fq archive using Trimmomatic but I get the following error:
Comand:
Output:
It seems there's something wrong with first line..
Thank you for helping
I am trying to trim low quality bases from a .fq archive using Trimmomatic but I get the following error:
Comand:
Code:
java -jar trimmomatic-0.36.jar SE -phred33 ORD0908_Forward.fq try.fq LEADING:3 TRAILING:3
Code:
TrimmomaticSE: Started with arguments: -phred33 ORD.fq try.fq LEADING:3 TRAILING:3 Automatically using 2 threads Exception in thread "main" java.lang.RuntimeException: Invalid FASTQ name line: >ORD at org.usadellab.trimmomatic.fastq.FastqParser.parseOne(FastqParser.java:68) at org.usadellab.trimmomatic.fastq.FastqParser.parse(FastqParser.java:164) at org.usadellab.trimmomatic.TrimmomaticSE.process(TrimmomaticSE.java:197) at org.usadellab.trimmomatic.TrimmomaticSE.run(TrimmomaticSE.java:306) at org.usadellab.trimmomatic.Trimmomatic.main(Trimmomatic.java:85) marta@martapc:~/Trimmomatic-0.36$
Thank you for helping
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