![]() |
|
![]() |
||||
Thread | Thread Starter | Forum | Replies | Last Post |
how does stranded and un-stranded quantification work? | weasteam | Bioinformatics | 2 | 05-10-2013 11:15 AM |
Mapping paired-end stranded RNA-seq data | hubin.keio | Bioinformatics | 3 | 03-28-2013 03:10 PM |
question about stranded RNA seq | StephaniePi83 | RNA Sequencing | 11 | 09-02-2012 05:25 PM |
RNA-Seq when not using the Illumina Kit | seqquest | Sample Prep / Library Generation | 1 | 10-07-2009 04:36 AM |
![]() |
|
Thread Tools |
![]() |
#1 |
Senior Member
Location: Mexico Join Date: Mar 2011
Posts: 137
|
![]()
Hi everyone,
I want to prepare several libraries for RNA-seq using the Illumina Hi-Seq. I am considering several kit options for making said libraries: Illumina's TruSeq Stranded Total RNA Kit [dUTP-based] Illumina's Directional Protocol [adaptation of small RNA-seq protocol, ligation] Epicentre's v2 RNA-Seq Library Preparation Kit [Tag/Block] Which of these libraries would you recommend for higher yields, greater accuracy, cost-effectiveness, etc? I would like to barcode each library individually, for which option 1 (Illumina TruSeq Stranded) seems like a good option, since the adapters are already supplied with 12 different indices. Looking forward to hearing your opinions. C |
![]() |
![]() |
![]() |
Tags |
directional, rna-seq, rnaseq, sequencing, stranded |
Thread Tools | |
|
|