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Old 06-04-2013, 11:46 AM   #1
carmeyeii
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Location: Mexico

Join Date: Mar 2011
Posts: 137
Default What kit would you recommend for Stranded RNA-Seq?

Hi everyone,

I want to prepare several libraries for RNA-seq using the Illumina Hi-Seq.

I am considering several kit options for making said libraries:

Illumina's TruSeq Stranded Total RNA Kit [dUTP-based]

Illumina's Directional Protocol [adaptation of small RNA-seq protocol, ligation]

Epicentre's v2 RNA-Seq Library Preparation Kit [Tag/Block]


Which of these libraries would you recommend for higher yields, greater accuracy, cost-effectiveness, etc?

I would like to barcode each library individually, for which option 1 (Illumina TruSeq Stranded) seems like a good option, since the adapters are already supplied with 12 different indices.

Looking forward to hearing your opinions.
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directional, rna-seq, rnaseq, sequencing, stranded

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